Worksheet Chapter 5: Metabolism and Enzymes

1. True or False: Anabolic reactions break down complex organic molecules and release energy in the form of ATP.

2. True or False: Heating an enzyme to 70°C lowers its kinetic energy, causing temporary inactivation.

3. Fill in the blank: The specific region of an enzyme molecule that binds with a complementary substrate molecule is called the ________ site.

4. Which enzyme is added to detergent to break down grease and oil stains from clothing?

5. State two general characteristics of enzymes.

6. Explain the Lock and Key Hypothesis of enzyme action.

7. An experiment was set up to measure starch digestion by salivary amylase at three temperatures:
- Tube A (0°C): Starch remains present after 30 minutes.
- Tube B (37°C): Starch disappears completely within 5 minutes.
- Tube C (80°C): Starch remains present after 30 minutes.
Explain the results obtained in Tube A and Tube C.

8. Enzymes play vital roles in everyday commercial industries.
(a) Name the enzyme used to produce lactose-free milk and explain how it functions.
(b) State one advantage of using immobilized enzymes in industrial food manufacturing.

9. Compare and contrast Anabolism and Catabolism.

10. Define the term 'activation energy' (Ea) in biochemical reactions.